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Research from the University of Alberta has identified the structure of the infectious prion protein, the cause of "mad cowdisease" or BSE, chronic wasting disease in deer and elk and Creutzfeldt-Jakob disease in humans, which has long remained a mystery.

The infectious prion protein is a misfolded protein, which makes it very difficult to purify and study. Since it clumps together, standard structural biology techniques cannot be used to study it. Since the protein was first purified in the 1980s researchers have made limited insights into the structure of the protein.

The collaborative study, used electron Cryomicroscopy to collect high-resolution electron micrographs. This was the first time this technology has been used on amyloid fibrils of the infectious prion, which are a special form of clumped-together proteins that form fibrils.

Holger Wille, co-principal investigator and an associate professor in the Department of Biochemistry at the University of Alberta's Faculty of Medicine & Dentistry, says, “The recent advances to electron cryomicroscopy technology are certainly a breakthrough.” we knew very little about the infectious prion protein and how it propagates. The use of these high-powered microscopes has finally given us some clarity.

The team had to develop a processing scheme for the data masses. There were thousands of electron micrographs, and they had to extract the best images. After three years of working, developing techniques and processing data the results in the paper is a three-dimensional model for the structure of the infectious prion protein.

The model can give insights into how the infectious prion protein propagates. The structure argues against existing theories of prion conversion and suggests how the process might actually work. The study suggests how infectious prions replicate by converting non-infectious, cellular versions into copies of themselves.

 

This study used model system prions, but they are now using the prions that infect cows (BSE), wild animals (Chronic Wasting Disease) and humans (Creutzfeldt - Jakob disease).